It is well-known that both macrophages and osteocytes are critical regulators of bone remodelling, yet there is limited understanding of the macrophage-osteocyte interaction, and how their crosstalk could affect mineralisation. This research aims to investigate the effects of macrophage polarisation on osteocyte maturation and mineralisation process. Indirect co-culture using conditioned medium was applied to investigate the impact of macrophages on osteocyte maturation and mineralisation. Our results identified that osteocytes were confined in an immature stage after the M1 macrophage stimulation, showing a more rounded morphology, higher expression of early osteocyte marker E11, and significantly lower expression of mature osteocyte marker DMP1. Surgically induced osteoarthritis (OA) rat model was used to investigate the macrophage-osteocyte interaction in inflammatory bone remodelling, as well as the involvement of the Notch signalling pathway in the mineralisation process. Immature osteocytes were found in inflammatory bone remodelling areas, showing altered morphology and mineralised structures similar to those observed under the stimulation of M1 macrophages in vitro, suggesting that M1 macrophages negatively affect osteocyte maturation, leading to abnormal mineralisation. The Notch signalling pathway was found to be down regulated in M1 macrophage-stimulated osteocytes as well as osteocytes in inflammatory bone. Overexpression of the Notch signalling pathway in osteocytes showed a significant circumvention on the negative effects from M1 macrophage. Taken together, our findings provide valuable insights into the mechanisms involved in abnormal bone mineralisation under inflammatory conditions.